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KMID : 0613820100200020213
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Journal of Life Science
2010 Volume.20 No. 2 p.213 ~ p.218
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Analysis of p53-Dependency of Differentially Expressed Genes by Capsaicin in Human Colorectal Cancer Cell
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Kim Hyo-Eun
Jang Min-Jung
Lim Seung-Hyun
Kim Hyo-Rim
Kim Soon-Young
Kim Jong-Sik
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Abstract
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In the present study, we investigated anti-proliferative activities of capsaicin and gene expression changes in response to capsaicin treatment in human colorectal HCT116 cells. The results showed that capsaicin decreased cell viabilities in a dose dependent manner and induced global gene expression changes. We found that 103 genes were up-regulated more than twofold, whereas 153 genes were down-regulated more than twofold by 100 μM capsaicin treatment. Among the up-regulated genes, we selected 4 genes (NAG-1, DDIT3, GADD45A and PCK2) and performed RT-PCR to confirm the microarray data. We found that 100 μM of capsaicin increased tumor suppressor p53 gene expression. In addition, the results showed that NAG-1, DDIT3 and GADD45A expressions were not dependent on p53 presence, whereas PCK2 expression. The results of this study may help to increase our understandings of the molecular mechanism of anti-proliferative activity mediated by capsaicin in human colorectal cancer cells.
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KEYWORD
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Capsaicin, anti-proliferative activity, gene expression, p53, human colorectal cancer
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